A simple method for long-term cryopreservation of Calonectria ilicicola on barley grains

摘要

We developed a simple method for long-term preservation of the soybean red crown rot fungus, Calonectria ilicicola, using barley grains. Autoclaved barley grains were inoculated with the fungus, then incubated at 25pC for 1 month. After incubation, grains were dried to approximately 3% moisture content, and stored at 4, 20, or 80pC for 3 years. C. ilicicola preserved on barley grains at 80pC remained viable without any change in mycelial growth and virulence. These results showed that C. ilicicola can be successfully cryopreserved for extended periods on barley grains at 80pC. We also confirmed that cultures preserved on barley grains are suitable for direct use without further manipulation as inocula in pathogenicity tests.