Multiplex polymerase chain reaction discriminates which eggplant isolates of Corynespora cassiicola are virulent to sweet pepper

摘要

Some isolates of Corynespora cassiicola from eggplant leaves with black blight can also cause Corynespora blight on sweet pepper. To differentiate eggplant isolates differing in virulence to sweet pepper, we first created two sets of primers based on nucleotide sequences of DNA fragments specific to virulent and to avirulent isolates, respectively, in a random amplified polymorphic DNA analysis using two random primers. A multiplex polymerase chain reaction using the two primer sets allowed us to discriminate isolates virulent to sweet pepper from among eggplant isolates.