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Analysis of DPPH inhibition and structure change of corn peptides treated by pulsed electric field technology

机译:脉冲电场技术处理玉米肽对DPPH的抑制作用和结构变化分析

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In this study, the effects on antioxidant activity and structure change of corn peptides (CPS) with 10 to 30 kDa molecular weight (MW) treated by pulsed electric field (PEF) technology were investigated. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) inhibition was used to evaluate the antioxidant activity of CPS. Response surface methodology (RSM) was used to investigate the effects of PEF treatment parameters on antioxidant activity of CPS. The optimal conditions were as follows: concentration of CPS 10 mg mL(-1), electric field intensity 15 kV cm(-1), and pulse frequency 2,000 Hz. Under the optimized conditions, the DPPH inhibition of CPS increased 32.1 %, compared to the sample untreated. And mid-infrared spectroscopy (MIR) was used for analyzing the structure change of CPS. The results showed that PEF technology could obviously increase the DPPH inhibition of CPS under the optimized conditions (P0.05).
机译:在这项研究中,研究了通过脉冲电场(PEF)技术处理的分子量为10至30 kDa的玉米肽(CPS)对抗氧化活性和结构变化的影响。 2,使用2-二苯基-1-吡啶并肼基(DPPH)抑制作用来评估CPS的抗氧化活性。响应面法(RSM)用于研究PEF处理参数对CPS抗氧化活性的影响。最佳条件如下:CPS的浓度为10 mg mL(-1),电场强度为15 kV cm(-1),脉冲频率为2,000 Hz。在最佳条件下,与未处理的样品相比,CPS的DPPH抑制增加了32.1%。并用中红外光谱(MIR)分析了CPS的结构变化。结果表明,在最佳条件下,PEF技术可以明显提高CPS对DPPH的抑制作用(P <0.05)。

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