Fast Separation Ultra-Performance Liquid Chromatography for Determination of Pre-Column Derivative Abamectin and Ivermectin Residues in Vegetable

摘要

A new residue method for quantification of abamectin and ivermectin in vegetable is described in the article. The derivative process is devised that acylating chemical is firstly performed by N-methylimidazole (MI) and trifluoroacetic anhydride (TFAA), then which reacted with hydroxyl function of abamerctin to make fluorescence. The influence of triethylamin (TEA) is examined. Separation is resolute by a short column of 1.7 mu m size and operated at high pressure values (10.000 psi). The optimal chromatographic condition and the highest sensitivity are achieved by acetonitrile: water (95: 5), 0.4 mL/min, 0.2 mu L injector. The detection limits of abamectin and ivermectin are 1 mu g/kg respectively.