The acute lethality and endocrine effect of 1,2,3,7,8-PeCDD in juvenile goldfish(Carassius auratus)in vivo

摘要

1,2,3,7,8-PeCDD was administrated to juvenile goldfish {Carassius auratus)by peritoneal injections to explore the acute lethality and endocrine effects of 1,2,3,7,8-PeCDD in vivo.The value of acute median lethal dosage(LD50)of 1,2,3,7,8-PeCDD was determined in acute lethality tests.The endocrine effect of 1,2,3,7,8-PeCDD,whose exposed concentrations were determined based on the LD50(1.84 mg/kg),was studied by measuring the plasma vitellogenin(Vtg)content in juvenile male goldfish with enzyme-linked immunosorbent assays(ELISA).Due to its significant induction of the plasma Vtg after one week's exposure in vivo in the 1/2 LD_(50)and LD_(30)groups,1,2,3,7,8-PeCDD might be one of the important contributors to the estrogenic effect of PCDDs in the environment.The values of 1/2 LD50 and LD30 were within the range of the effective dosages of 1,2,3,7,8-PeCDD,indicating that there was a certain relationship between the estrogenic effective dosages and the LD_(50).