Participation of ADP/ATP- and Aspartate/GIutamate Antiporters in the Uncoupling Action of Fatty Acids in Liver Mitochondria of the Frog Rana temporaria

摘要

Data are presented on molecular mechanisms of uncoupling of oxidative phosphorylationby fatty acids (laurate) in liver mitochondria of one of the poikilothermal animals,the frog Rana temporaria.It has been shown that the uncoupling action of laurate in frog liver mitochondria,like in those of mammals,occurs with participation of protein carriers of anions of the inner mitochondrial membrane,ADP/ATP- and aspartate/glutamate antiporters.At the same time,in frog liver mitochondria the uncoupling activity of laurate is lower than in liver mitochondria of mammals (white mice).Seasonal differences in the laurate uncoupling activity in frog liver mitochondria are revealed:it is much lower in April,than in January,the season of metabolic depression.This difference is due to that in January the degree of participation of the aspartate/glutamate antiporter in the uncoupling is considerably decreased.