The gamma-delta T-cell cytokine response to peritonitis

摘要

The inflammatory cytokine response of peritoneal and splenic gamma delta T-cells and macrophages to polymicrobial bacterial peritonitis was investigated. The production of intracellular Tumor Necrosis Factor-alpha (TNF) and Interleukin-10 (IL-10) was measured using flow cytometry at 6, 24, and 48 hrs following cecal ligation and puncture or sham laparotomy. TNF-alpha production was induced in peritoneal - but not splenic - gamma delta T-cells in response to both sham surgery and peritonitis. gamma delta T-cells demonstrated no IL-10 production. Peritoneal and splenic macrophages demonstrated earlier TNF and IL-10 responses to CLP than gamma delta T-cells. We conclude that gamma delta T-cells are local mediators of inflammation.