首页> 外文期刊>Journal of enzyme inhibition and medicinal chemistry. >In vitro effects of some drugs on catalase purified from human skin.
【24h】

In vitro effects of some drugs on catalase purified from human skin.

机译:某些药物对从人皮肤纯化的过氧化氢酶的体外作用。

获取原文
获取原文并翻译 | 示例
           

摘要

Catalase enzyme (H202: oxidoreductase; E.C. 1.11.1.6) was purified from human skin homogenate using ammonium sulfate precipitation and DEAE-Sephadex A50 ion exchange chromatography at 4 degrees C and some characteristics of the enzyme were investigated. The human skin enzyme, having a specific activity of 1354.5 EU/mg proteins was purified with a yield of 43.13% and 1110-fold. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed a single band for the enzyme. Inhibition by piroxicam, ketoprofen, diclofenac sodium, sulfamethoxazole and nidazole occurred with I50 values of 0.414, 1.29, 1.8, 3.83, and 8.64 mM, respectively.
机译:使用硫酸铵沉淀和DEAE-Sephadex A50离子交换色谱法在4摄氏度下从人皮肤匀浆中纯化过氧化氢酶(H202:氧化还原酶; E.C. 1.11.1.6),并研究了该酶的某些特性。纯化具有1354.5 EU / mg蛋白比活的人皮肤酶,产率为43.13%和1110倍。十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)显示该酶有一条条带。吡罗昔康,酮洛芬,双氯芬酸钠,磺胺甲恶唑和硝唑的抑制作用的I50值分别为0.414、1.29、1.8、3.83和8.64 mM。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号