首页> 外文期刊>Journal of Endodontics: Official Journal of American Association of Endodontists >The antioxidant property of pachymic acid improves bone disturbance against AH plus-induced inflammation in MC-3T3 E1 cells
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The antioxidant property of pachymic acid improves bone disturbance against AH plus-induced inflammation in MC-3T3 E1 cells

机译:chy酸的抗氧化特性改善了针对MC-3T3 E1细胞中AH +引起的炎症的骨骼干扰

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Introduction: The cytotoxicity of resin-based sealer is influential on the inflammatory reaction and cell survival for oral periapical cells. In this study, pachymic acid as an antioxidant was investigated for the improvement of bone disturbance against AH Plus (Dentsply DeTrey GmbH, Konstanz, Germany)-induced inflammation in MC-3T3 E1 cells. Methods: AH Plus was prepared according to the manufacturer's instructions. Using mouse osteoblast cells (MC-3T3 E1), a specimen of AH Plus was eluted with the culture medium for 1 day and was diluted by 30%. The cellular cytotoxicity and reactive oxygen species formation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and 2′,7′-dichlorodihydrofluorescein diacetate with fluorescence-activated cell sorting. The secretion of proinflammatory cytokines was determined by an enzyme-linked immunosorbent assay, and the expression of inflammatory and osteogenic molecules was determined by immunoblotting. Results: Cells with AH Plus elutes showed a decrease of cell viability and ALP activity. However, pachymic acid and N-acetyl-L-cysteine (control antioxidant) restored cell viability and ALP activity damaged by AH plus. The secretion of nitric oxide, tumor necrosis factor α, and interleukin-1β were increased in AH Plus-stimulated MC-3T3 E1 cells, but pachymic acid suppressed its production. Furthermore, pachymic acid reduced the receptor activator of nuclear factor-κB ligand, cyclooxygenase-2, matrix metalloproteinase-2 and -9, increased bone morphogenetic protein-2 and -7, and runt-related transcription factor 2 despite AH Plus stimuli. In addition, pachymic acid affected the removal effect of reactive oxygen species formation as did N-acetyl-L-cysteine. More importantly, pachymic acid inhibited nuclear factor-κB translocation. Conclusions: The property of pachymic acid can mitigate the unfavorable conditions induced by AH Plus stimuli. Therefore, the use of pachymic acid is suggested to prevent the complications of oral diseases such as inflammation and alveolar destruction of the oral cavity.
机译:简介:树脂基封闭剂的细胞毒性影响口腔根尖周细胞的炎症反应和细胞存活率。在这项研究中,对作为抗氧化剂的pa酸进行了研究,以改善针对AH Plus(Dentsply DeTrey GmbH,德国康斯坦茨)引起的MC-3T3 E1细胞炎症的骨干扰。方法:AH Plus根据制造商的说明进行制备。使用小鼠成骨细胞(MC-3T3 E1),用培养基洗脱AH Plus样品1天,并稀释30%。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物和2',7'-二氯二氢荧光素二乙酸酯通过荧光激活细胞分选确定细胞的细胞毒性和活性氧的形成。通过酶联免疫吸附测定确定促炎细胞因子的分泌,并通过免疫印迹确定炎性和成骨分子的表达。结果:带有AH Plus洗脱液的细胞显示细胞活力和ALP活性降低。但是,厚朴酸和N-乙酰基-L-半胱氨酸(对照抗氧化剂)恢复了被AH +破坏的细胞活力和ALP活性。一氧化氮,肿瘤坏死因子α和白介素-1β的分泌在AH Plus刺激的MC-3T3 E1细胞中增加,但厚朴酸抑制了其产生。此外,尽管有AH Plus刺激,但厚朴酸降低了核因子-κB配体,环氧合酶2,基质金属蛋白酶2和-9的受体活化剂,骨形态发生蛋白2和-7以及与矮子相关的转录因子2的表达。另外,厚朴酸与N-乙酰基-L-半胱氨酸一样影响活性氧物种形成的去除效果。更重要的是,草酸抑制了核因子-κB的移位。结论:厚朴酸的性质可以缓解AH Plus刺激引起的不利条件。因此,建议使用草酸来预防口腔疾病的并发症,例如炎症和口腔的肺泡破坏。

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