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Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: An in vitro study

机译:三种抗生素组合的细胞毒性作用和抗菌功效:一项体外研究

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Introduction: A 3-antibiotic combination (3Mix) is widely used in endodontics for root canal disinfection, particularly in pulp revascularization procedures. However, the cytotoxicity of 3Mix has not been evaluated. The purpose of this study was to determine the cytotoxicity and antibacterial efficacy of 3Mix and each single antibiotic component of 3Mix. Methods: For the cytotoxicity test, human dental pulp cells and apical papilla cells were exposed to either 3Mix or to each single antibiotic component of 3Mix using concentrations of 0.024, 0.097, 0.39, 1.56, 6.25, and 25.00 μg/mL for 1, 3, 5, and 7 days. Cell viability was determined using the 3-[4,5-dimethylthiazol-2- yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. For the antibacterial test, 25.00 μg/mL and 0.39 μg/mL 3Mix or single antibiotic were tested on bacteria isolated from necrotic teeth by measuring bacterial recovery on blood agar. Results: The 0.024-μg/mL concentration of all experimental groups generated the highest dental pulp cell or apical pulp cell viability at all time periods. On day 7, 0.39 μg/mL 3Mix produced more than 90% cell viability; 25.00 μg/mL 3Mix completely eliminated isolated bacteria, whereas 0.39 μg/mL was unable to eradicate all bacteria. However, the overall bacterial reduction was significantly different compared with the control group (P < .01). Conclusions: All drugs except metronidazole induced cytotoxicity on cultured cells. 3Mix generated higher cytotoxicity compared with a single drug. The cytotoxicity increased in a concentration- and time-dependent manner; 0.39 μg/mL 3Mix had less cytotoxicity and was able to significantly reduce bacteria isolated from necrotic teeth.
机译:简介:3-抗生素组合(3Mix)被广泛用于根管消毒的牙髓治疗中,尤其是在牙髓血运重建过程中。但是,尚未评估3Mix的细胞毒性。这项研究的目的是确定3Mix和3Mix的每种抗生素成分的细胞毒性和抗菌功效。方法:为进行细胞毒性测试,将人牙髓细胞和根尖乳头细胞暴露于3Mix或3Mix的每种单一抗生素成分中,浓度分别为0.024、0.097、0.39、1.56、6.25和25.00μg/ mL,分别为1、3 ,5和7天。使用3- [4,5-二甲基噻唑-2-基] -2,5-二苯基溴化四唑(MTT)测定法测定细胞活力。对于抗菌测试,通过测量血琼脂上的细菌回收率,对从坏死牙齿中分离出的细菌测试了25.00μg/ mL和0.39μg/ mL的3Mix或单一抗生素。结果:所有实验组的浓度为0.024-μg/ mL,在所有时间段内均产生最高的牙髓细胞或顶端牙髓细胞活力。在第7天,0.39μg/ mL 3Mix产生了90%以上的细胞活力; 25.00μg/ mL 3Mix完全消除了分离出的细菌,而0.39μg/ mL无法消灭所有细菌。但是,与对照组相比,总体细菌减少量有显着差异(P <.01)。结论:除甲硝唑外的所有药物均可对培养细胞产生细胞毒性。与单一药物相比,3Mix产生更高的细胞毒性。细胞毒性以浓度和时间依赖性方式增加。 0.39μg/ mL 3Mix具有较低的细胞毒性,能够显着减少从坏死牙齿中分离出的细菌。

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