A novel method of tissue collection and storage: validation using SELDI-TOF MS analysis.

摘要

To the Editor: Bouamrani et al. (1) recently reported an approach to SELDI-TOF mass spectrometry that uses direct apposition of cryosections onto pro-teinchips to enrich spectral profiles and improve the discriminatory power of the technique. To prevent protein degradation, however, samples must be stored frozen and locally processed or shipped frozen to a proteomics facility. We have found that tissue can be preserved on filter paper in a way that maintains the protein profiles obtained with frozen tissues and allows targeted tissue analysis and storage at room temperature.