首页> 外文期刊>Journal of Economic Entomology >Efficacy of Cry1Ac:Cry1F Proteins in Cotton Leaf Tissue Against Fall Armyworm, Beet Armyworm, and Soybean Looper (Lepidoptera: Noctuidae)
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Efficacy of Cry1Ac:Cry1F Proteins in Cotton Leaf Tissue Against Fall Armyworm, Beet Armyworm, and Soybean Looper (Lepidoptera: Noctuidae)

机译:棉叶组织中Cry1Ac:Cry1F蛋白对秋季粘虫,甜菜粘虫和大豆弯Loop的功效(鳞翅目:夜蛾科)

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摘要

Cotton, Gossypium hirsutum L., plants expressing Cry1Ac and Cry1F insecticidal crystal proteins of Bacillus thuringiensis Berliner (Bt) were evaluated against selected lepidopteran pests including fall armyworm, Spodoptera frugiperda (J. E.-Smith), beet armyworm, Spodoptera exigua (Hubner), and soybean looper, Pseudoplusia includens (Walker). Studies were conducted in a range of environments, challenging various cotton tissue types from several varieties containing a combination of Cry1Ac and Cry1F proteins. In fresh tissue bioassays of mature leaves and squares (flower buds) and in artificial field infestations of white flowers, plants containing Cry1Ac:Cry1F significantly reduced levels of damage (leaf defoliation, bract feeding, penetrated squares and bolls, and boll abscission) and induced significantly greater mortality (90-100%) of fall armyworm compared with that on non-Bt cotton plants. Plants containing Cry1Ac:Cry1F conferred high levels (100%) of soybean looper mortality and low levels (0.2%) of leaf defoliation compared with non-Bt cotton. Beet armyworm was relatively less sensitive to Cry1Ac:Cry1F cotton plants compared with fall armyworm and soybean looper. However, beet armyworm larval development was delayed 21 d after infestation (DAI), and ingestion of plant tissue was inhibited (14 and 21 DAI) on the Cry1Ac:Cry1F plants compared with that on non-Bt cotton plants. These results show Cry1Ac:Cry1F cotton varieties can be an effective component in a management program for these lepidopteran pest species. Differential susceptibility of fall armyworm, beet armyworm, and soybean looper larvae to Cry1Ac:Cry1F cotton reinforces the need to sample during plant development and respond with a foliar insecticide if local action thresholds are exceeded.
机译:对棉花,棉铃虫,表达苏云金芽胞杆菌柏林(Bt)的Cry1Ac和Cry1F杀虫晶体蛋白的植物进行了抗药性评估,评估了其鳞翅目害虫包括秋天的夜蛾,斜纹夜蛾(Sodoptera frugiperda)(JE-Smith),甜菜夜蛾,甜菜夜蛾(Spodoptera exigua)(Hubner),大豆弯角,Pseudoplusia includens(Walker)。在一系列环境中进行了研究,挑战了包含Cry1Ac和Cry1F蛋白组合的几个变种的各种棉花组织类型。在成熟的叶子和正方形(花蕾)的新鲜组织生物测定中以​​及在白色花朵的人工田间侵染中,含有Cry1Ac:Cry1F的植物显着降低了伤害水平(叶片脱叶,片饲喂,穿透正方形和铃虫以及铃铃脱落)并诱导与非转基因棉花相比,秋夜蛾的死亡率显着提高(90-100%)。与不含Bt的棉花相比,含有Cry1Ac:Cry1F的植物的大豆弯腰mortality死亡率高(100%),叶片脱叶率低(0.2%)。与秋天的夜蛾和大豆套and相比,甜菜夜蛾对Cry1Ac:Cry1F棉株的敏感性相对较低。然而,甜菜夜蛾幼虫的发育被延迟了21天,与非Bt棉花相比,Cry1Ac:Cry1F植物的植物组织的摄入被抑制了(14和21 DAI)。这些结果表明,Cry1Ac:Cry1F棉花品种可以作为这些鳞翅目害虫物种管理计划的有效组成部分。秋季夜蛾,甜菜夜蛾和大豆loop的幼虫对Cry1Ac:Cry1F棉的敏感性不同,这就增加了在植物发育过程中进行采样的必要性,如果超过了局部作用阈值,则应使用叶面杀虫剂进行响应。

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