The protein kinase CK2 inhibitor TBB mediates up-regulation of MEK3/6 and p38delta activities, down-regulation of ERK1/2 activity and induction of G1/S arrest in normal human epidermal autocrine proliferating keratinocytes.

摘要

Protein kinase CK2 (previously called casein kinase 2) (EC 2.7.11.1) is an extremely conserved serine/threonine kinase ubiquitously distributed in mammalian cells, where typically is found as a tetramer, consisting of two catalytic subunits (a and/or a') and a dimer of non-catalytic (3 subunit [1]. CK2 is strongly distinguished from others protein kinases by particularly three properties - high constitutive activity, lack of an acute mechanism/ s of regulation and extreme pleiotropy (with list of over 300 substrates). Mounting evidence supports the importance of the enzyme in diverse biological processes, especially its essential role in cell signal regulation [2]. Although, the involvement of CK2 in cell signaling is vast-investigated, very little is known about how CK2 participates in the signal control of epidermal keratinocyte physiology.