Evaluation of the subgingival microbiota of alcoholic and non-alcoholic individuals.

摘要

OBJECTIVES: The aim of this study was to evaluate the composition of the subgingival microbiota of alcoholic and non-alcoholic individuals. METHODS: The study was conducted with 49 alcoholic and 49 non-alcoholic males of the Philippe Pinel Institute, Rio de Janeiro, Brazil. The subjects were selected by convenience and two criteria were used to diagnose alcohol dependence: the CAGE (cut-down, annoyed, guilt, eyes-opener) questionnaire and the International Statistical Classification of Diseases, 10th edition (WHO). Subgingival biofilm samples were obtained from 4 sites, 2 with probing depth (PD) >/= 4 mm and 2 sites with PD < 4 mm. The presence and levels of 45 bacterial taxa were analysed using the checkerboard technique. RESULTS: The prevalence of bacterial species was not significantly different between groups. However, alcoholics showed significantly higher mean counts of Aggregactibacter actinomycetemcomitans, Fusobacterium nucleatum nucleatum, and Porphyromonas gingivalis (adjusted p < 0.001). Moreover, alcoholics harboured significantly higher mean levels of Capnocytophaga sputigena, Fusobacterium nucleatum vincentii, F. nuc. nucleatum, Gemella morbillorum, Neisseria mucosa, P. gingivalis, Streptococcus gordonii, and Tannerella forsythia at sites with PD < 4 mm or >/= 4 mm compared to non-alcoholics (p /= 4 mm of non-alcoholics (p