Quantification of monomer elution and carbon-carbon double bonds in dental adhesive systems using HPLC and micro-Raman spectroscopy.

摘要

OBJECTIVES: To quantify monomer elution from different adhesive systems using reverse-phase high-performance liquid chromatography (HPLC) and correlate this elution with the ratio of carbon-carbon double bonds from monomer to polymer (RDB) obtained using micro-Raman spectroscopy. METHODS: Thirty dentine discs were cut from 30 human, intact, third molars and randomly allocated to five groups according to the adhesive applied: total-etch, Excite (Ivoclar Vivadent), two-bottle self-etch, Clearfil SE (Kuraray), one-bottle self-etch, Clearfil 3S (Kuraray), ormocer-based, Admira (Voco) and Filtek Silorane adhesive system (FS) (3M ESPE). Monomer elution was studied 1h, 6h, 24h, 96h and 7 days after immersion in 75% ethanol/water. The RDB was calculated immediately after light-curing and thereafter at 24h and 7 days. The data were statistically analysed using one-way ANOVA and Pearson's correlation coefficient (p<0.05). RESULTS: More than 90% of the whole elution occurred during the first 1h, except for BisGMAin FS, with the highest absolute amount from Clearfil SE and the highest wt% from Admira. Initial RDB was in the ascending order FS