Dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cells.

摘要

This study investigated the ability of some dairy bacteria to bind concanavalin A (Con A), peanut agglutinin (PNA) and jacalin (AIL) in vitro, preventing their toxicity towards mouse intestinal epithelial cells (IEC). Con A and AIL significantly reduced IEC viability in vitro, as determined by Trypan Blue dye exclusion or by propidium iodide/fluorescein diacetate/Hoescht staining. Different strains of dairy bacteria were able to remove lectins from the media. 2 strains were subjected to treatments used to remove S-layer, cell wall proteins, polysaccharides and lectin-like adhesins. They were then assayed for the ability to bind dietary lectins and reduce toxicity against IEC and to adhere to IEC after interaction with lectins. Con A and AIL were removed by Propionibacterium acidipropionici and P. freudenreichii by binding with specific sugar moieties on the bacterial surface. Removal of lectins by bacteria impaired IEC protection. Adhesion of P. acidipropionici to IEC was reduced but not abolished after binding of Con A or AIL. Results suggest that removal of Con A or AIL by dairy propionibacteria is an effective means of avoiding their toxic effects on colonic cells in vitro, and that consumption of foods containing these bacteria could be used as a tool to protect the intestinal epithelia.