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Lysine decarboxylase of Vibrio parahaemolyticus: kinetics of transcription and role in acid resistance

机译:副溶血性弧菌赖氨酸脱羧酶:转录动力学及其在耐酸中的作用

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Aim: The aim of this study was to investigate the detailed mechanisms of acid resistance in Vibrio parahaemolyticus. Methods and Results: All 11 strains of V. parahaemolyticus survived lethal acidic conditions following acid adaptation, and accumulation of cadaverine was detected. The addition of lysine improved survival, suggesting that lysine decarboxylase plays a role in the adaptive acid tolerance response. Two open reading frames (ORF) in V. parahaemolyticus, which are separated by a noncoding region, were found to be highly homologous to bacterial lysine decarboxylase (cadA) and lysine/cadaverine antiporter (cadB) genes. Transcriptional analyses of this operon revealed acid induction and enhanced induction by external lysine. The relative expression ratio of each transcript was found to follow the trend of cadA mRNA > cadB mRNA > cadBA bi-cistronic mRNA. A mutated strain, with a disrupted cadA gene, showed attenuated acid survival. Conclusions: We identified the lysine decarboxylase gene operon of V. parahaemolyticus. Expression of this operon was induced under acidic conditions. The cadA-mutated strain constructed in this study showed weaker tolerance to acidic conditions than the wild-type strain. Significance and Impact of the Study: Vibrio parahaemolyticus utilizes the lysine decarboxylation pathway for survival in acidic conditions.
机译:目的:本研究的目的是研究副溶血弧菌的抗酸性的详细机制。方法和结果:11株副溶血性弧菌在酸适应后均在致命的酸性条件下存活,并检测了尸胺的积累。赖氨酸的添加提高了存活率,表明赖氨酸脱羧酶在适应性酸耐受性反应中起作用。发现副溶血性弧菌中的两个开放阅读框(ORF)与非编码区分开,与细菌赖氨酸脱羧酶(cadA)和赖氨酸/尸胺反转运蛋白(cadB)基因高度同源。该操纵子的转录分析显示酸诱导和外部赖氨酸诱导增强。发现每个转录本的相对表达率遵循cadA mRNA> cadB mRNA> cadBA双顺反子mRNA的趋势。带有cadA基因破坏的突变菌株显示出弱的酸存活率。结论:我们鉴定了副溶血弧菌的赖氨酸脱羧酶基因操纵子。该操纵子的表达是在酸性条件下诱导的。在这项研究中构建的cadA突变菌株对酸性条件的耐受性比野生型菌株弱。研究的意义和影响:副溶血性弧菌利用赖氨酸脱羧途径在酸性条件下存活。

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