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Comparison of biochemical and molecular methods for the identification of bacterial isolates associated with failed loggerhead sea turtle eggs

机译:比较生化和分子方法鉴定与失败的海龟卵有关的细菌分离株

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Aims: Comparison of biochemical vs molecular methods for identification of microbial populations associated with failed loggerhead turtle eggs. Methods and Results: Two biochemical (API and Microgen) and one molecular methods (16s rRNA analysis) were compared in the areas of cost, identification, corroboration of data with other methods, ease of use, resources and software. The molecular method was costly and identified only 66% of the isolates tested compared with 74% for API. A 74% discrepancy in identifications occurred between API and 16s rRNA analysis. The two biochemical methods were comparable in cost, but Microgen was easier to use and yielded the lowest discrepancy among identifications (29%) when compared with both API 20 enteric (API 20E) and API 20 nonenteric (API 20NE) combined. A comparison of API 20E and API 20NE indicated an 83% discrepancy between the two methods. Conclusions: The Microgen identification system appears to be better suited than API or 16s rRNA analysis for identification of environmental isolates associated with failed loggerhead eggs. Significance and Impact of the Study: Most identification methods are not intended for use with environmental isolates. A comparison of identification systems would provide better options for identifying environmental bacteria for ecological studies.
机译:目的:比较生化和分子方法鉴定与失败的龟卵有关的微生物种群。方法和结果:比较了两种生化方法(API和Microgen)和一种分子方法(16s rRNA分析)在成本,鉴定,与其他方法的数据确证,易用性,资源和软件方面。分子方法成本高昂,仅能鉴定出66%的分离物,而API仅为74%。 API和16s rRNA分析之间的识别差异为74%。两种生化方法的成本相当,但是与API 20肠溶性(API 20E)和API 20非肠溶性(API 20NE)组合使用相比,Microgen更易于使用,并且在鉴定中的差异最小(29%)。 API 20E和API 20NE的比较表明,两种方法之间的差异为83%。结论:Microgen鉴定系统似乎比API或16s rRNA分析更适合用于鉴定与失败的鱼相关的环境分离株。研究的意义和影响:大多数鉴定方法均不适用于环境分离物。鉴定系统的比较将为鉴定用于生态研究的环境细菌提供更好的选择。

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