Evaluation of novel fluorogenic substrates for the detection of glycosidases in Escherichia coli and enterococci

Perry JD; James AL; Morris KA; Oliver M; Chilvers KF; Reed RH; Gould FK

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Evaluation of novel fluorogenic substrates for the detection of glycosidases in Escherichia coli and enterococci

机译：评价用于检测大肠杆菌和肠球菌中糖苷酶的新型荧光底物

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Aims: Enzyme substrates based on 4-methylumbelliferone are widely used for the detection of Escherichia coli and enterococci in water, by detection of beta-glucuronidase and beta-glucosidase activity respectively. This study aimed to synthesize and evaluate novel umbelliferone-based substrates with improved sensitivity for these two enzymes.

机译：目的：基于4-甲基伞形酮的酶底物通过分别检测β-葡萄糖醛酸酶和β-葡萄糖苷酶的活性，被广泛用于检测水中的大肠杆菌和肠球菌。这项研究旨在合成和评估新型的基于伞形酮的底物，对这两种酶具有更高的敏感性。

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来源
《Journal of applied microbiology 》 |2006年第5期| 共9页
作者
Perry JD; James AL; Morris KA; Oliver M; Chilvers KF; Reed RH; Gould FK;
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正文语种 eng
中图分类微生物学 ;
关键词
enterococci; enzyme substrates; Escherichia coli; glucosidase; glucuronidase; umbelliferones; TOTAL COLIFORMS; WATER SAMPLES; PHOSPHATASES; GLUCOSIDASE; ENUMERATION; TECHNOLOGY; ASSAY;

机译：肠球菌;酶底物;大肠杆菌;葡萄糖苷酶;葡萄糖醛酸酶;伞形酮;总大肠菌群;水样;磷酸酶;葡糖苷酶;计数;技术;测定;

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专利

1. Evaluation of novel fluorogenic substrates for the detection of glycosidases in Escherichia coli and enterococci [J] . Perry JD, James AL, Morris KA, Journal of applied microbiology . 2006 ,第5期

机译：评价用于检测大肠杆菌和肠球菌中糖苷酶的新型荧光底物
2. Semi-automated fluorogenic PCR assays (TaqMan) forrapid detection of Escherichia coli O157:H7 and other shiga toxigenic E. coli. [J] . Sharma VK, Dean Nystrom-EA, Casey TA Molecular and Cellular Probes: The Location, Diagnosis and Monitoring of Disease by Specific Molecules and Cell Lines . 1999 ,第4期

机译：半自动荧光PCR分析（TaqMan）可快速检测大肠杆菌O157：H7和其他志贺毒素性大肠杆菌。
3. Off-on fluorogenic substrate harnessing ESIPT and AIE features for in situ and long-term tracking of β-glucuronidase in Escherichia coli [J] . Wei Xianhu, Wu Qingping, Feng Ying, Sensors and Actuators . 2020 ,第Feba期

机译：利用ESIPT和AIE功能的脱机荧光底物可原位和长期跟踪大肠杆菌中的β-葡萄糖醛酸苷酶
4. 8-hydroxyquinoline-glucuronide sodium salt used as electroactive substrate for a sensitive voltammetric detection of Escherichia coli in water samples [C] . Jorg Ettenauer, Karen Zuser, Karlheinz Kellner, Eurosensors Conference . 2017

机译：8-羟基喹啉 - 葡糖醛钠钠盐用作电活性基质，用于水样中大肠杆菌的敏感伏安检测
5. Development and evaluation of a gas sensor-based instrument for the detection and differentiation of Escherichia coli O157:H7 from non-O157:H7 E. coli. [D] . Younts, Spring Marie. 1999

机译：开发和评估一种基于气体传感器的仪器，用于检测和区分O157：H7大肠杆菌和非O157：H7大肠杆菌。
6. Evaluation of a fluorogenic assay for detection of Escherichia coli in foods. [O] . B J Robison 1984

机译：评价用于检测食品中大肠杆菌的荧光检测法。
7. Evaluation of a fluorogenic assay for detection of Escherichia coli in foods [O] . B J Robison 1984

机译：评价荧光测定检测食品中大肠杆菌的荧光测定
8. Results of the Interlaboratory Testing Study for the Comparison of Methods for Detection and Enumeration of Enterococci and Escherichia Coli in Combined Sewer Overflows (CSOs) [R] . 2009

机译：综合下水道溢流（CsOs）中肠球菌和大肠杆菌检测和计数方法比较的实验室间测试研究结果

Evaluation of novel fluorogenic substrates for the detection of glycosidases in Escherichia coli and enterococci

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