Capture probe conjugated to paramagnetic nanoparticles for purification of Alexandrium species (Dinophyceae) DNA from environmental samples

Galluzzi L; Bertozzini E; del Campo A; Penna A; Bruce IJ; Magnani M

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Capture probe conjugated to paramagnetic nanoparticles for purification of Alexandrium species (Dinophyceae) DNA from environmental samples

机译：捕获探针与顺磁性纳米颗粒偶联，用于从环境样品中纯化亚历山大藻属物种（Dinophyceae）DNA

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摘要

Aims: To develop a rapid, cost-effective and selective Alexandrium DNA extraction procedure from environmental samples in order to provide good-quality template for the downstream PCR-based detection assay.

机译：目的：开发一种从环境样品中快速，经济高效且选择性的亚历山大DNA提取方法，以便为基于PCR的下游检测提供高质量的模板。

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来源
《Journal of applied microbiology 》 |2006年第1期| 共8页
作者
Galluzzi L; Bertozzini E; del Campo A; Penna A; Bruce IJ; Magnani M;
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正文语种 eng
中图分类微生物学 ;
关键词
Alexandrium; capture probe; detection; paramagnetic nanoparticles; PCR; 5.8S rDNA; POLYMERASE-CHAIN-REACTION; C VIRUS-RNA; PHASE HYBRIDIZATION; SEQUENCE ALIGNMENT; PCR; DINOFLAGELLATE; SENSITIVITY; POLIOVIRUS; PARTICLE; MINUTUM;

机译：亚历山大藻;捕获探针;检测;顺磁性纳米粒子;PCR;5.8S rDNA;聚合酶链反应;C病毒-RNA;相杂交;序列比对;PCR;二氟甲磺酸钠;敏感性;脊髓灰质炎病毒;颗粒;MINUTUM;

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专利

1. Capture probe conjugated to paramagnetic nanoparticles for purification of Alexandrium species (Dinophyceae) DNA from environmental samples [J] . Galluzzi L, Bertozzini E, del Campo A, Journal of applied microbiology . 2006 ,第1期

机译：捕获探针与顺磁性纳米颗粒偶联，用于从环境样品中纯化亚历山大藻属物种（Dinophyceae）DNA
2. Molecular phylogeny of three hitherto unreported Alexandrium species: Alexandrium hiranoi, Alexandrium leei and Alexandrium satoanum (Gonyaulacales, Dinophyceae) inferred from the 18S and 26S rDNA sequence data [J] . Kim KY, Yoshida M, Kim CH Phycologia . 2005 ,第4期

机译：从18S和26S rDNA序列数据推断出的三个迄今未报道的亚历山大藻物种的分子系统发育：亚历山大·希拉诺伊（Alexandraium hiranoi），亚历山大·亚历山大雷（Alexanderium leei）和亚历山大·亚历山大（Alexandium satoanum）（Gonyaulacales，Dinophyceae）
3. Formal Revision of the Alexandrium tamarense Species Complex (Dinophyceae) Taxonomy: The Introduction of Five Species with Emphasis on Molecular-based (rDNA) Classification [J] . John Uwe, Litaker R. Wayne, Montresor Marina, Protist . 2014 ,第6期

机译：亚历山大属植物群（Dinophyceae）分类的正式修订：强调基于分子（rDNA）分类的五种物种
4. Automated high-throughput purification of genomic DNA from whole blood using Promega's MagneSil~(TM) paramagnetic particles with either the Max Yield or MagneSil~(TM) ONE normalized purification methods [C] . Rex M. Bitner, Susan C. Koller, Jacqui Sankbeil Society of Photo-Optical Instrumentation Engineers Conference on Microarrays and Combinatorial Technologies for Biomedical Applications :Design, Fabrication, and Analysis . 2003

机译：使用Promega的镁〜（TM）顺磁颗粒从全血的自动化高通量纯化，MAX产量或磁头杆菌〜（TM）一种标准化纯化方法
5. Development of Chemical Methods for Oligonucleotide Purification, Paramagnetic Labeling and Synthesis of Dna-Based Advanced Materials [D] . He, Muhan. 2021

机译：寡核苷酸纯化化学方法的发展，顺磁标记和基于DNA的先进材料的合成
6. Formal Revision of the Alexandrium tamarense Species Complex (Dinophyceae) Taxonomy: The Introduction of Five Species with Emphasis on Molecular-based (rDNA) Classification [O] . Uwe John, R. Wayne Litaker, Marina Montresor, -1

机译：亚历山大他玛伦种（Dinophyceae）分类学的正式修订：强调基于分子（rDNA）分类的五种物种的介绍
7. Discrimination of Three Highly Toxic Alexandrium tamarense (Dinophyceae) Isolates Using FITC-conjugated Lectin Probes [O] . E. S. Cho 2001

机译：使用FITC缀合的凝集素探针辨别三种高度毒性亚历山大葡萄串（Dinophyceae）分离物
8. Analysis of Toxic and Non-Toxic Alexandrium (Dinophyceae) Species Using RibosomalRNA Gene Sequences [R] . Scholin, C. A. 1993

机译：利用核糖体RNa基因序列分析毒性和非毒性亚历山大藻（Dinophyceae）种

Capture probe conjugated to paramagnetic nanoparticles for purification of Alexandrium species (Dinophyceae) DNA from environmental samples

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