Study on the binding equilibrium between surfactant FC-134 and DNA by resonance light scattering technique

摘要

This article presents the study of binding equilibrium of fluoro surfactant FC-134 to DNA was studied by resonance light scattering. It was found that the interaction resulted in strongly enhanced resonance light scattering signals characterized by the RLS peak at 370nm. The binding constant Kf and the number of binding n were calculated according to the equilibrium equation, which was established based on the data of the resonance light scattering measurements at 370 nm. It was found that the binding Kf and the number of binding n were 5.98×105 and 1.4, respectively.