Evaluation of different chromatographic techniques for resolution of pigments in extract from annatto (Bixa orellana L.) seeds

摘要

Chromatographic techniques such as paper, column and thin-layer (TLC) were employed for resolving pigments of annatto seeds. The TLC technique on a silica gel G layer in a solvent system consisting of chloroform, ethanol and acetic acid (80:2:1, v/v) gave the best performance. Up to 11 components were resolved from chloroformic extract of the seeds.