Microparticle-loaded neonatal porcine Sertoli cells for cell-based therapeutic and drug delivery system

摘要

Neonatal porcine Sertoli cells (NPSC) are immune privileged cells showing innate phagocytic and antibacterial activities. NPSC have been shown capable of immunoaltering the body's response and possess lung homing capacity. These properties encourage investigation of NPSC as functional components of cell-based therapeutic protocols to treat lung infections and related complications. In this work, for the first time, NPSC were tailored to carry an antibiotic drug loaded into poly(D,L lactic) acid microparticles (MP). A loading protocol was developed, which afforded 30% drug uptake and high stability over time,with little or no effects on NPSC viability,morphology, reactive oxygen species production and DNA integrity. FSH receptor integrity, and TGFβ (transforming growth factor β) and AMH (anti-Müllerian hormone) expressionswere unchanged after 1 month of cryopreservation. Protein tyrosine kinase activation due to phagocytosis may have had resulted in changes in inhibin B expression. The activity ofMP-loaded or NPSC alone against Pseudomonas aeruginosa was maintained throughout 1 month of storage. NPSC couple an innate antibacterial activitywith the capacity to embody drug loadedMP.We showed for the first time that engineered NPSC can be cryopreserved with no loss of their basic properties, thereby possibly representing a novel approach for cell-based therapeutic and drug delivery system.