Liquid chromatography: Is it essential for the determination ofarginine and methylated arginines by tandem mass spectrometry?

摘要

Our primary aim was to develop a faster, simpler procedure forestimating MAs in samples from patients with chronic kidney diseases as part of an on-going clinical study. In this group of patients’ concentrations of MAs are known to be significantly higher than in healthy individuals. For example mean concentrations of SDMA were reported to be 5.5 times higher in patients with renal disease than in a healthy control group [4]. In haemodialysis patients the concentrations have been reported to be further increased [5]. We required a relatively simple method involving as few stages as possible to measure in particular ADMA and SDMA at the higher concentrations found in renal patients. To this end a compromise was reached accepting less precise measurements at low “normal” concentrations in favour of a faster less complex method suitable for routine use in a hospital laboratory.