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Peptide profiling in epithelial tumor plasma by the emerging proteomic techniques

机译:新兴的蛋白质组学技术在上皮肿瘤血浆中进行肽谱分析

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The plasma peptide component (PPC) from ten melanoma (Mel), breast cancer (BC) and healthy individuals was examined by a combination of RP-HPLC, surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOFMS) and tandem mass spectrometry. A three peak pattern (2023, 2039, 2053.5 m/z) was primarily observed in melanoma. Two peaks (2236.1 and of 2356.3 m/z) were found only in BC samples. Fibrinogen alpha and inter-alpha-trypsin inhibitor heavy chain H4 fragments were absent in both tumor samples. (c) 2005 Elsevier B.V. All rights reserved.
机译:通过RP-HPLC,表面增强激光解吸/电离飞行时间质谱(SELDI-TOFMS)的组合检查了十个黑素瘤(Mel),乳腺癌(BC)和健康个体的血浆肽成分(PPC)和串联质谱。在黑素瘤中主要观察到三个峰型(2023、2039、2053.5 m / z)。仅在BC样品中发现了两个峰(2236.1和2356.3 m / z)。在两个肿瘤样品中均不存在纤维蛋白原α和间α-胰蛋白酶抑制剂重链H4片段。 (c)2005 Elsevier B.V.保留所有权利。

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