首页> 外文期刊>Journal of chromatography, B. Analytical technologies in the biomedical and life sciences >Simultaneous determination of barbiturates in human biological fluids by direct immersion solid-phase microextraction and gas chromatography–mass spectrometry
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Simultaneous determination of barbiturates in human biological fluids by direct immersion solid-phase microextraction and gas chromatography–mass spectrometry

机译:直接浸没固相微萃取和气相色谱-质谱法同时测定人体生物液中的巴比妥类药物

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摘要

Simultaneous determination of seven barbiturates in human whole blood and urine by combining direct immersion solid-phase microextraction (DI–SPME) with gas chromatography-mass spectrometry (GC–MS) is presented. The main parameters affecting the DI–SPME process, such as SPME fibers, salt additives, pHs, extraction temperatures and immersion times were optimized for simultaneous determination of the drugs. The extraction efficiencies were 0.0180–0.988 and 0.0156–2.76% for whole blood and urine, respectively. The regression equations of the drugs showed excellent linearity for both samples; the correlation coefficients (r~2) were 0.994–0.999. The detection limits for whole blood were 0.05–1 μg ml~(-1), and those for urine 0.01–0.6 μg ml~(-1). Actual quantitation could be made for pentobarbital in whole blood and urine obtained from volunteers, who had been orally administered a therapeutic dose of the drug. The DI–SPME/GC–MS procedure for barbiturates established in this study is simple and sensitive enough to be adopted in the fields of clinical and forensic toxicology.
机译:提出了将直接浸入式固相微萃取(DI-SPME)与气相色谱-质谱(GC-MS)结合同时测定人全血和尿液中的七个巴比妥酸盐的方法。影响DI–SPME工艺的主要参数,例如SPME纤维,盐添加剂,pH,提取温度和浸泡时间均经过优化,可同时测定药物。全血和尿液的提取效率分别为0.0180–0.988和0.0156–2.76%。两种药物的回归方程均显示出极好的线性。相关系数(r〜2)为0.994-0.999。全血的检出限为0.05–1μgml〜(-1),尿液的检出限为0.01–0.6μgml〜(-1)。可以对从口服治疗剂量药物的志愿者获得的全血和尿液中的戊巴比妥进行实际定量。这项研究中建立的巴比妥类药物的DI-SPME / GC-MS程序非常简单,灵敏,足以在临床和法医毒理学领域采用。

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