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Determination of gabapentin in human plasma by capillary electrophoresis with laser-induced fluorescence detection and acetonitrile stacking technique

机译:毛细管电泳-激光诱导荧光检测-乙腈堆积技术测定人血浆中的加巴喷丁

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摘要

A sensitive analytical method for gabapentin [1-(aminomethyl) cyclohexaneacetic acid] (GBP) in human plasma based on capillary electrophoretic separation and laser-induced fluorescence (LIF) detection has been developed. 6-Carboxyfluorescein succinimidyl ester (CFSE) was used for precolumn derivatization of the non-fluorescent drug in plasma. Optimal separation and detection were obtained with an electrophoretic buffer of 50 mM sodium borate (pH 9.5) and an air-cooled argon-ion laser (excitation at 488 nm, emission at 520 nm). A calibration curve ranging from 0.3 to 150 μM was shown to be linear. The concentration limit of detection (LOD) in plasma was 60 nM. We also demonstrate how the detection limit can be enhanced by using acetonitrile stacking technique. With stacking, the limit of detection for gabapentin in plasma was 4.8 nM. A calibration curve ranging from 0.03 to 15 μM was shown to be linear. Both the within-day and day-to-day reproducibility and accuracy were ≤10.8 and 6.0%, respectively.
机译:建立了一种基于毛细管电泳分离和激光诱导荧光(LIF)检测的人血浆中加巴喷丁[1-(氨基甲基)环己烷乙酸](GBP)的灵敏分析方法。 6-羧基荧光素琥珀酰亚胺酯(CFSE)用于血浆中非荧光药物的柱前衍生。使用50 mM硼酸钠(pH 9.5)的电泳缓冲液和空冷的氩离子激光器(在488 nm激发,在520 nm发射)可实现最佳分离和检测。 0.3至150μM的校准曲线显示为线性。血浆中检测的浓度极限(LOD)为60 nM。我们还演示了如何通过使用乙腈堆积技术来提高检测限。堆积后,血浆中加巴喷丁的检出限为4.8 nM。 0.03至15μM的校准曲线显示为线性。每日和每天的重现性和准确性分别≤10.8和6.0%。

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