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Determination of amino acids in fodders and raw materials using capillary zone electrophoresis

机译:毛细管区带电泳法测定饲料和原料中的氨基酸

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Two schemes were offered for analysis of amino acid contents in fodders and raw materials for mixed fodders by capillary zone electrophoresis (CZE). The first variant provides express analysis of four technologically important amino acids (lysine, methionine, threonine, cystine) in borate buffer on characteristic absorption of aminogroup (190 nm), with limits of quantitation being on average 0.2%. The second scheme includes pre-capillary derivatization of amino acids using phenylisothiocyanate (PITC) and separation of phenylthiocarbamyl (PTC)-derivatives obtained by CZE with a detection on 254 nm, which allows to widen a list of detectable components up to 19 (without tryptophan) and significantly improve detection limits down to 0.01%. Acid hydrolysis was used for a sample preparation. The results of analysis of fodders were compared using such methods, as CZE, ion exchange chromatography (amino acid analyzer) and reversed-phase (RP)-HPLC (with gradient technique of elution).
机译:提供了两种方案通过毛细管区带电泳(CZE)分析饲料和混合饲料原料中的氨基酸含量。第一个变体提供了对硼酸盐缓冲液中四个技术上重要的氨基酸(赖氨酸,蛋氨酸,苏氨酸,胱氨酸)的氨基特征吸收(190 nm)的明确分析,定量限平均为0.2%。第二种方案包括使用苯基异硫氰酸酯(PITC)对氨基酸进行预毛细血管衍生化,以及通过CZE在254 nm处检测分离出苯硫代氨基甲酸酯(PTC)衍生物,这使得可检测组分的范围扩大到19种(无色氨酸) ),并将检出限大幅降低至0.01%。酸水解用于样品制备。使用CZE,离子交换色谱法(氨基酸分析仪)和反相(RP)-HPLC(采用梯度洗脱技术)等方法比较了饲料的分析结果。

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