A simple method was developed for determining florfenicol concentration in a small volume (250 μl) of plasma from five phylogenetically diverse species of freshwater fish. Florfenicol was isolated from the plasma matrix through C_(18) solid-phase extraction and quantified by reversed-phase high-performance liquid chromatography with UV detection. The accuracy (84-104%), precision (%RSD ≤ 8), and sensitivity (quantitation limit <30 ng/ml) of the method indicate its usefulness for conducting pharmacokinetic studies on a variety of freshwater fish.
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机译：开发了一种简单的方法来测定五种系统发育多样的淡水鱼中小剂量（250μl）血浆中氟苯尼考的浓度。通过C_（18）固相萃取从血浆基质中分离出氟苯尼考，并通过具有UV检测的反相高效液相色谱进行定量。该方法的准确度（84-104％），精确度（％RSD≤8）和灵敏度（定量限<30 ng / ml）表明其可用于对各种淡水鱼进行药代动力学研究。
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