H-POINT STANDARD ADDITIONS METHOD FOR RESOLUTION OF OVERLAPPING CHROMATOGRAPHIC PEAKS WITH DIODE ARRAY DETECTION BY USING AREA MEASUREMENTS - DETERMINATION OF PHENOL AND CRESOLS IN WATERS

摘要

The H-point standard additions method (HPSAM) is proposed to resolve overlapped peaks in liquid chromatography. The method uses the areas obtained at two previously selected wavelengths as analytical signals, and good results are obtained for highly overlapped peaks with highly overlapped spectra. The principal benefits of the method are the ease with which the required wavelengths can be selected, its insensitivity to changes in the retention time of the peak from one injection to other, and the possibility of using it in highly or only partially overlapped peaks, We have applied it to phenol and cresol determinations and the highly overlapped peak of m- and p-cresol is resolved by this method.