MICELLAR LIQUID CHROMATOGRAPHY OF PLANT GROWTH REGULATORS DETECTED BY DERIVATIVE FLUOROMETRY

摘要

A micellar liquid chromatographic method with fluorimetric detection was developed for the determination of the plant growth regulators indol 3-yl acetic acid (IAA), 2-(1-naphthyl) acetic acid (I-NAA), indol 3-yl propionic acid (IPA), 2-(2-naphthyl) acetic acid (2-NAA), indol 3-yl butyric acid (IBA), 2-(1-naphthyl) acetamide (1-Namide) and indol 3-yl acetic acid ethyl ester (IAA ethyl ester). Extraction of plant hormones was performed with methanol, followed by purification with ethyl acetate, solid-phase extraction and again partitioning against diethyl ether. Sodium dodecylsulphate (10 mM) is used as mobile phase to elute the compounds in a maximum run-time of 23 min. Partially or unresolved peaks are separated by calculation of the 1st derivative chromatogram. Detection limits are between 0.30 mu g g(-1) and 1.10 mu g g(-1).