CHROMATOGRAPHY OF BOVINE LACTOSERUM ON THE IMMOBILIZED METALLIZED DYE DRIMARENE RUBINE R/K 5BL - MODULATION OF SELECTIVITY BY METAL EXCHANGE AND APPLICATION TO PURIFICATION OF MILK IMMUNOGLOBULINS

摘要

Chromatography on immobilized Drimarene Rubine R/K 5-BL, a copper-containing reactive dye, was evaluated as a method of purifying immunoglobulins from bovine lactoserum. Varying the eluent composition did not provide pure immunoglobulins. However, changing the metal from copper to zinc allowed the preparation of essentially pure immunoglobulins, in quantitative yield, using imidazole in 2 M NaCl as mobile phase. This procedure can probably be adapted to the very large-scale preparation of immunoglobulins from milk which are administered orally for human and animal therapy.