MIXED ION-PAIR HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY OF URIDINE 5'-DIPHOSPHO-ALPHA-D-GLUCURONIC ACID AND ITS HYDROLYSIS PRODUCTS

摘要

Although conventional ion-exchange HPLC and ion-pair reversed-phase HPLC using tetrabutylammonium hydroxide allowed ready determination of uridine 5'-diphospho-alpha-D-glucose and its hydrolysis products, neither method was suitable for the determination of uridine 5'-diphospho-alpha-D-glucuronic acid (alpha-UDPGA). However, mixed ion-pair reversed-phase HPLC, using a combination of tetrabutylammonium hydroxide and tetraethylammonium hydroxide (1:1), has been found to offer a convenient and effective means of simultaneously determining alpha-UDPGA and its hydrolysis products, UDP, UMP and cUMP. [References: 6]