POSTCOLUMN CHEMILUMINESCENCE, ULTRAVIOLET AND EVAPORATIVE LIGHT-SCATTERING DETECTORS IN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF TRIACYLGLYCEROL OXIDATION

摘要

Postcolumn chemiluminescence (CL), ultraviolet (UV) and evaporative light-scattering (ELS) detectors were compared in the HPLC of triacylglycerol (TAG) oxidation products. Polar lipid fractions from autoxidized TAG samples were first separated from non-polar material by solid-phase extraction and then analysed by RP-HPLC in combination with UV-CL and UV-ELS detectors. TAG hydroperoxides were detected with a CL detector, oxidation products containing conjugated diene structures with a UV detector and all non-volatile oxidation products with an ELS detector. More accurate characterization of the oxidation products was possible by combining the information from different detectors. For samples at early stages of oxidation, the CL and UV detectors were more suitable because of their better sensitivity and selectivity compared with the ELS detector.