THIN-LAYER CHROMATOGRAPHIC SEPARATION OF ENANTIOMERIC DANSYLAMINO ACIDS USING A MACROCYCLIC ANTIBIOTIC AS A CHIRAL SELECTOR

摘要

The enantiomeric resolution of ten dansyl-DL-amino acids was achieved on thin silica gel plates impregnated with a macrocyclic antibiotic, erythromycin, as the chiral selector. Different combinations of 0.5 M aqueous NaCl-MeCN-MeOH were found to be successful in resolving various dansyl-DL-amino acids. Spots were detected using a fixed dual-wavelength (254 nm) ultraviolet chamber.