Immobilized liposome chromatography of drugs on capillary continuous beds for model analysis of drug-membrane interactions

摘要

Liposomes were immobilized in capillary continuous beds with covalently linked C_4 or C_8 alkyl ligands for chromatographic analysis of drug interaction with phospholipid bilayers, as reflected by drug retention volumes and calculated differences in interaction free energies. This procedure is a high-resolution micro-scale version of immobilized liposome chromatography for prediction of diffusion of drugs across biological membranes. The logarithm of the specific capacity factors of several structurally unrelated drugs showed a linear correlation with the logarithm of known apparent drug permeabilities through Caco-2 epithelial cell monolayers. The latter values are used for prediction of absorption of orally administered drug doses.