REVERSED-PHASE AFFINITY CHROMATOGRAPHY OF ECDYSTEROIDS WITH BORONIC ACID-CONTAINING ELUENTS

摘要

Selective effects of boronic acid-containing eluents on the chromatographic behaviour of ecdysteroids are described. The use of such eluents provides a simple, rapid and sensitive means to assess the presence of certain diol groups in ecdysteroids by ordinary RP-HPLC. It is demonstrated that ecdysteroids with different sets of diols in their molecules have individual shapes of chromatographic peaks. A mechanism of the boronic acid effect is discussed.