CURRENT STATUS AND PERSPECTIVES OF COMPUTER-AIDED TWO-DIMENSIONAL DENSITOMETRY [Review]

摘要

Two-dimensional electrophoresis is a powerful technique for demonstrating cellular changes in protein synthesis and post-translational modification. The method of computer-aided two-dimensional densitometry was developed to facilitate the comparison of gels. However, powerful minicomputers were required to execute the sophisticated programs in the early stages of development. Recent advances in workstations have given rise to commercially available desktop systems on which the full functions of two-dimensional densitometry including spot matching and database construction are available. Thus two-dimensional gel protein databases of various cell types have been accumulating, and some of them are accessible through ExPASy server on the Internet computer network. [References: 35]