RELATIONSHIP BETWEEN THE HYDROPHOBICITY AND SPECIFIC HYDROPHOBIC SURFACE AREA OF PESTICIDES DETERMINED BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY COMPARED WITH REVERSED-PHASE THIN-LAYER CHROMATOGRAPHY

摘要

The hydrophobicity and specific hydrophobic surface area of 12 commercial pesticides have been determined by reversed-phase high-performance liquid chromatography (RP-HPLC) using octadecyl-bonded silica support and water-methanol mixtures as eluents. The pesticides showed regular retention behaviour, their log k' values decreased linearly with increasing concentration of methanol in the eluent. The hydrophobicity and specific hydrophobic surface area of pesticides were significantly intercorrelated, although the chemical structures of the pesticides were different. Principal component analysis proved that the hydrophobicity parameters determined by RP-HPLC and reversed-phase thin-layer chromatography are slightly different and herbicides and fungicides could not be distinguished according to their hydrophobicity parameters. This indicates that differences in the biological activity of these pesticides cannot be attributed to either their hydrophobicity or specific hydrophobic surface area alone.