PREPARATIVE ISOLATION OF THE EUTOMER OF CYCLOTHIAZIDE BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY ON A CELLULOSE-DERIVED CHIRAL STATIONARY PHASE WITH TOLUENE-ACETONE AS THE MOBILE PHASE

摘要

The enantiomeric pair containing the eutomer of cyclothiazide can be resolved by HPLC on commercially available cellulose-derived chiral stationary phases (CSPs). However, the poor solubility of cyclothiazide in solvents compatible with this kind of CSP makes this separation difficult on a preparative scale. This operation was achieved with a CSP constituted by a 3,5-dimethylphenylcarbamate/10-undecenoate derivative of cellulose fixed on allylsilica gel. This CSP is resistant to all the polar solvents usually applied in HPLC.