DETERMINATION OF ALENDRONATE SODIUM BY ION CHROMATOGRAPHY WITH REFRACTIVE INDEX DETECTION

摘要

Refractive index (RI) detection is applied to the ion chromatographic OC) determination of alendronate sodium, an important drug for the treatment of bone diseases, using a Waters IC-Pak HR anion-exchange column with dilute nitric acid (6 mM) as the mobile phase. The method is validated and demonstrated to be accurate, precise, specific and robust. It is a good, alternative method for routine assay vs. the IC-indirect UV method. The detection limit (4 . 10(-4) mg/ml) is comparable to or better than that of the IC-indirect UV detection (1 . 10(-3) mg/ml). The mechanism of the IC separation was explored by the application of the Regnier retention model and van't Hoff analysis.