APPLICATION OF A COMPARATIVE EVALUATION OF SEVERAL REVERSED-PHASE COLUMNS TO THE AUTOMATED ANALYSIS OF CANDIDATE PHARMACEUTICALS

摘要

This report discusses the evaluation of several modern reversed-phase chromatographic packing materials, and describes the application of a selected column in a method used to screen the purities of pharmaceutical candidates and intermediates. Columns are first evaluated with an acetonitrile gradient using a test mixture containing acids, bases and compounds of widely differing polarities. Selected phases are further assessed by analysing many typical samples. Finally, the use of the chosen column and method are described and discussed. [References: 6]