RESOLUTION OF PROMETHAZINE, ETHOPROPAZINE, TRIMEPRAZINE AND TRIMIPRAMINE ENANTIOMERS ON SELECTED CHIRAL STATIONARY PHASES USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

摘要

The separation of the enantiomers of three phenothiazines and a dibenzazepine were investigated on several different chiral stationary phases without the use of derivatization or column switching. The phenothiazines selected were promethazine, ethopropazine and trimeprazine and the dibenzazepine was trimipramine. Three classes of columns were studied for their ability to separate the enantiomers of these compounds: brush or Pirkle, inclusionary, and affinity types. The columns studied were (alpha-R-naphthyl)ethylurea, (S)-tert. -leucine-(R)-1-(alpha-naphthyl) ethylamine, dinitrobenzoylphenylgycine; beta-cyclodextrin, beta-acetyylated cyclodextrin; gamma-cyclodextrin; Chiralcel-OD; Chiralcel-OJ and Ovomucoid. Changes in mobile phase composition were studied on each column to determine the effects of solvents on the separation of the various enantiomers. Simplex calculations for mobile phase variations were not performed because they do not predict elution order reversals. [References: 15]