QUANTITATIVE DETERMINATION OF ORGANIC SOLVENTS BY CAPILLARY ELECTROPHORESIS USING INDIRECT UV DETECTION

摘要

A sodium dodecyl sulphate (SDS)-based micellar electrokinetic capillary chromatography method is reported for the quantitative determination of a range of simple organic solvents employing indirect UV absorbance detection. Veronal buffer is used to provide both pH buffering and the background UV signal for indirect detection. The concentrations of both SDS and veronal buffer were optimized using an experimental design approach. The method was shown to give acceptable performance in terms of various performance parameters including selectivity, linearity, precision and detection limits. The method was succesfully applied to the determination of the ethanol content in pharmaceutical formulations and alcoholic beverages. Good correlation was obtained between the CE results and the label claims. The method has several features including simplicity, short analysis time and robustness. [References: 17]