High-performance liquid chromatography-electrospray lonization -mass spectrometry study of ginkgolic acid in the leaves and fruits of the ginkgo tree (Ginkgo biloba)

摘要

A method is developed for qualitative analysis of ginkgolic acids in the leaves and fruits of Ginkgo biloba by high -performance liquid chromatography (HPLC)-electrospray ionization-mass spectrometry technique. Negative ionization mode is successful in obtaining a very abundant deprotonated molecule [M - H]-. The mass detection sensitivity is higher than ultraviolet detection but relies heavily on the concentration of acetic acid in the HPLC eluent, which consists of acetonitrile-water-acetic acid. The method is also very specific of the analysis of ginkgolic acid with no interferences from the sample matrix.