Determination of Suifamethazine Residue in Chicken Serum and Egg by High-Performance Liquid Chromatography with Chemiluminescence Detection

摘要

A sensitive method that involves high-performance liquid chromatography with cliemiluminescence detection is developed for the determination of sulfamethazine residue in samples of chicken serum and egg. Sulfamethazine is extracted from the samples and derivatized with fluorescamine. The derivatized samples are eluted by reversed-phase chromatography using a mixture of 10 mM potassium phosphate and 30% acetonitrih- on a Nova-Pak C_(18) column. The post-column reagents in the chemiluminescence systemare 1mM bis[2-(3,6,9-trioxadecanyloxycarbonyl)-4-nitrophenyl] oxalate and 0.3M hydrogen peroxide in aceionilrile. The detection limit in the standard solution is 1 ng/mL, and the calibration curve is linear between 1 and 100 ng/mL. The recoveries of spiked samples (50 ppb) are 95.8 ± 9.7% in chicken serum samples and 84.9 ± 10.7% in egg samples. In actual sample analysis, the lowest detectable concentration of sulfamethazine (0.073 mu g/mL) was found at 48 h in serum and on day 9 (0.017 mu g/g) in egg after oral administration of a dose of 100 mg/kg body weight to hens.