THIN-LAYER CHROMATOGRAPHIC DETECTION OF DICHLORVOS AND DIMETHOATE USING ORCINOL

摘要

A sensitive and selective thin-layer chromatographic method for the detection of dichlorvos and dimethoate using orcinol is described. The alkali hydrolysis product of dichlorvos or dimethoate with orcinol produces a yellow fluorescent compound. The reagent does not react with other organophosphorus, organochlorine and carbamate insecticides. The constituents of viscera (amino acids, peptides, proteins, etc.) and plant materials do not interfere with the test. The fluorescence detection limits for dichlorvos and dimethoate are ca. 1 mu g per spot (1.40 mu g/cm(2)) and ca. 15 mu g per spot (21 mu g/cm(2)), respectively. [References: 10]