PURIFICATION OF ALPHA-L-ARABINOFURANOSIDASE USING A SINGLE-COLUMN MINI-SCALE ISOELECTRIC FOCUSING UNIT

摘要

Fabrication of a single-tube mini-scale isoelectric focusing unit with useful modifications is described. Provision of a side-arm to the unit facilitated easy collection of protein samples without disturbing the density and pH gradients. An ion-conducting polymer containing ether and hydroxy groups was fixed at the anodic end of the unit that separated the anodic solution from other solutions and also provided an easy path for charge transport. Using this unit, alpha-L-arabinofuranosidase (EC 3.2.1.55) was purified from crude broth of Sclerotium rolfsii in three steps with 33-fold purification. [References: 4]