Anion-exchane high--erformance liquid chromatography assays of plasma lipoproteins and mofified low-density lipoproteins using a ProtEx-DEAE column

摘要

Previously [Anal. Biochem., 232 (1995) 163—17 1], we reported a high-performance liquid chromatography (HPLC) assay method for human plasma lipoproteins using a diethylaminoethyl (DEAE)-glucomannan column, which is not commercially available. In this study, HPLC assay methods for lipoproteins in plasma samples of human and experimental animals, andProtEx-DEAE column. For the assays of plasma lipoprotems, the method includes complete separation of bigh-denstty lipoproteins, LDLs and very low-density lipoproteuns within 20 mm using stepwise eXution. and determination by post-column reaction with an enzymatic cholesterol reagent as the total cholesterol (TC) level. Similarly, mild oxidative and artificially oxidised LDLs were separated into their subtractions using scepwise elutiori, and determined based on the TC level. The methods using the DEAE-glucomannan and Pro~.Ex-DEAE columns were cross-validated. There was an excellent correlation between the two methods.