Determinationofurinary methylated purine pattern by high-performance liquid chromatography

摘要

We describe the group selective separation and quantification of unmodified and modified purines in human urine by high-performance reverse phase liquid chromatogiaphy. The pattern of oxypurines and methylated purines: hypoxanthine (Hx), xanthine (X), 1-methyl hypoxanthine (1-MHx~, 1-methyl guanine (1-MG), 3-methyl guanine (3-MG), 7-methyl guanine (7-MG), 1-methyl xanthine (1-MX). 3-r~hy1 xanthine (3-MIX. 7-methyl xanthine (7-MX), 1,7-dimethyl guanine (1,7-dMG), 1,3-dimethyl xanthine (1,3-dMX), 1,1-diax~thyl xanihine (3.7-dMX) and 1,3,7-trimethyl xanthine (l,3,7-tMX) were determined in a single run in urine of a healthy subject and a gout patient before and after treatment with allopurmol. This method may be useful to investigate the urimy ptern of methylated bases in diseases involving purine metabolism.